1 00:00:00,930 --> 00:00:02,470 My name is Caye Johnson. 2 00:00:02,470 --> 00:00:06,380 I went to work at NASA Ames in 1964. 3 00:00:06,380 --> 00:00:07,820 I was a biologist in the 4 00:00:07,820 --> 00:00:10,300 Life Detection Branch and I worked in 5 00:00:10,300 --> 00:00:13,170 the Lunar Biological Laboratory which was 6 00:00:13,170 --> 00:00:15,850 specifically designed and built 7 00:00:15,850 --> 00:00:17,560 to look for microbial life 8 00:00:17,560 --> 00:00:19,850 in the Apollo 11 samples. 9 00:00:19,850 --> 00:00:22,690 So in order to go into the lab, we would have to 10 00:00:22,690 --> 00:00:25,810 walk through an air shower, where everything would 11 00:00:25,810 --> 00:00:27,720 get blown off of us. 12 00:00:27,720 --> 00:00:29,170 You know we had to be really careful that 13 00:00:29,170 --> 00:00:32,770 we didn't introduce a microbe into the samples 14 00:00:32,770 --> 00:00:35,400 and then falsely say that we'd found it. 15 00:00:35,400 --> 00:00:37,970 We were culturing the samples 16 00:00:37,970 --> 00:00:40,890 under 300 different environments. 17 00:00:40,890 --> 00:00:44,220 And there were ten petri dishes for each environment. 18 00:00:44,220 --> 00:00:46,930 Typically the way you make petri dishes 19 00:00:46,930 --> 00:00:49,210 is you have agar with different kinds of 20 00:00:49,210 --> 00:00:51,250 chemicals in it, depending upon what it is 21 00:00:51,250 --> 00:00:52,690 you're trying to culture. 22 00:00:52,690 --> 00:00:54,570 In one of the sequences, you can see the guy 23 00:00:54,570 --> 00:00:56,920 sort of running like a little pump and then 24 00:00:56,920 --> 00:00:58,810 he's going from petri dish to petri dish 25 00:00:58,810 --> 00:01:01,430 and that's just putting a certain amount of agar 26 00:01:01,430 --> 00:01:02,320 in each petri dish. 27 00:01:02,320 --> 00:01:04,900 You would dump the sample in, like there, so that 28 00:01:04,900 --> 00:01:07,680 it falls onto this disk right underneath 29 00:01:07,680 --> 00:01:09,000 where the funnel is. 30 00:01:09,000 --> 00:01:12,690 And then you see them attach a hose 31 00:01:12,690 --> 00:01:15,570 and that hose is high pressure gas. 32 00:01:15,570 --> 00:01:18,200 Scatters it, so that you get more uniform 33 00:01:18,200 --> 00:01:21,040 distribution of the lunar sample over the surface 34 00:01:21,040 --> 00:01:22,730 of the petri dish. 35 00:01:22,730 --> 00:01:25,220 And then, you would take the petri dish and put it 36 00:01:25,220 --> 00:01:27,170 underneath the microscope. 37 00:01:27,170 --> 00:01:30,610 We only looked at the petri dishes twice a week. 38 00:01:30,610 --> 00:01:32,120 You know, sometimes it would be me, 39 00:01:32,120 --> 00:01:34,540 sometimes it would be some of the other women. 40 00:01:34,540 --> 00:01:37,600 And that's how we examined the petri dishes 41 00:01:37,600 --> 00:01:41,920 to see if there was anything growing on them. 42 00:01:41,920 --> 00:01:44,700 No signs of life were ever found 43 00:01:44,700 --> 00:01:48,160 in the lunar samples. 44 00:01:48,160 --> 00:01:51,050 The research paved the way for later work 45 00:01:51,050 --> 00:01:54,100 in astrobiology and the search for life on Mars. 46 00:01:54,100 --> 00:02:03,165